Fig. 1

Viability assay using different MitA concentrations and influence on radiosensitivity. A Viability of GBM cell lines. HROG05 and HROG63 were treated with MitA for 1 × 72 h [red dots] or 2 × 72 h [blue dots] in ascending concentrations [2–100 nM], respectively. n = 3–7 independent experiments. Mean + SEM; IC₂₀ and IC₅₀ values are marked on the y-axis. B IC₅₀ calculation for MitA after single (72 h) or double (2 × 72 h) treatment with ( +) or without (−) radiation. C Spectral flow cytometry. Analysis was done on HROG02 and GBM03 cells to dissect to potential differences in treatment response between ultra-low passage and long-term cultured cells. Mean + SD, n = 3 independent experiments. Two-way ANOVA (Tukey’s multiple comparison test)