Fig. 2

Effects of MitA on cellular radiosensitivity. A GBM cells were treated with increasing concentrations [2–100 nM] for 24 h before irradiation with 2 Gy (IBL637) and subsequent medium change [light purple] vs. non-irradiated MitA-treated cells [dark purple] for 2 × 72 h. Quantitative analyses were done using Calcein-AM assay. n = 3–7 independent experiments. *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control, Two-way ANOVA (Tukey’s multiple comparison test). B Representative images of 2D-cultured GBM cells (HROG02, HROG05, HROG63) stained with anti-H2A.X Phospho (Ser139) [red], treated with 5 nM MitA or left untreated with [ +] and without [−] irradiation. Scale bar as indicated: 50 µm. Nuclei were counterstained with DAPI. Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope. Representative images of n = 3 independent experiments