Fig. 1

Selection of potential candidate genes and analysis of clinical parameter correlations and survival. A Schematic diagram of the analytic protocol for the selection of sorafenib-mediated candidate genes. The 4 selected candidate genes were consistently observed in these two datasets. B The gene descriptions and fold changes in the GSE14520 and GSE94550 datasets are shown. C–D, J–K KM survival curves (OS and RFS) of 2 groups of patients with HCC grouped by AGR2 expression level [cutoff established on the basis of Roessler liver microarray scores] (C-D, GSE14520) and the cycle threshold value (Ct) obtained by quantitative PCR in our collected HCC specimens (J–K, median, 39-dCt). Patients with high AGR2 levels have worse overall survival and recurrence-free survival. E–I The AGR2 levels with several parameter correlations are shown. L, M Immunohistochemistry staining showing AGR2 expression in HCC specimens (200X). AGR2 is highly expressed in tumor tissues compared with normal liver tissue. d: delta