Fig. 2

Sorafenib decreases cell viability, increases cell apoptosis and induces AGR2 secretion in HCC. A–D The viability of J7, Hep3B, HepG2, and Huh7 HCC cells treated with 5 and 10 μM sorafenib for 24–48 h was examined using MTT assay. E–H Cell apoptosis was determined in HepG2 and Huh7 cells after stimulation with 5 and 10 μM sorafenib. The quantification of apoptotic cells is shown in F, H. Sorafenib decreases cell viability and increases cell apoptosis in HCC cells. I–R AGR2 RNA I–L and protein M–R levels, both intracellular M–P and extracellular Q, R, were examined via RT–PCR and Western blotting after 5 and 10 μM sorafenib treatment for 24–48 h. Sorafenib induces AGR2 secretion from the cytosol into conditioned medium rather than exerting transcriptional or translational regulation (lane 1, 4:untreatment; lane 2, 3, 5, 6:sorafenib treatment). Ponceau S was used as an internal control