Fig. 4

Sorafenib regulates ER stress-related molecules. A–E The protein A and RNA B, C, D, E levels of ER stress-related molecules were examined by Western blotting A and RT–PCR B, C, D, E after treatment of HepG2 A, D, E (left), B and Huh7 A, D, E (right), C cells with 5 μM and 10 μM sorafenib with or without AGR2 silencing D and 60 ng/ml recombinant AGR2 (rAGR2) stimulation E. Sorafenib induces the dysregulation of several ER stress-related molecules. AGR2 is involved in sorafenib-induced XBP-1 splicing. C: cleavage; u: unspliced; s: spliced; siNC: vector control siRNA; siAGR2: AGR2 siRNA. 0: untreatment; 5, 10: sorafenib treatment