Fig. 7

AGR2 is involved in the sorafenib-regulated IRE1α-XBP1 cascade. A, B, C, D The RNA C, D and protein A, B levels of the ER stress-related molecules p-IRE1α, IRE1α A, B, and XBP1 (u and s, C, D) were examined via Western blotting A, B and RT–PCR C, D after AGR2 silencing D and treatment with sorafenib at concentrations ranging from 0 to 10 μM in both parental (PC) and sorafenib-resistant (SR) HepG2 (A, (C, D, left)) and Huh7 (B, (C, D, right)) cells. Sorafenib-induced p-IRE-1α, IRE-1α regulation and XBP-1 splicing in sorafenib-sensitive cells are attenuated in sorafenib-resistant cells. u: unspliced; s: spliced; siNC: negative control siRNA, siRNA vector only; siAGR2: AGR2 siRNA. 0: untreatment; 7, 10: sorafenib treatment