Fig. 7
From: A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis
IGF2BP1 is the target gene of miR-27b-3p/miR-31-5p regulated by circRRM2. Alterations in the miR-27b-3p and IGF2BP levels in MDA-MB-231 (A) or BT-549 (B) cells following miR-27b-3p overexpression. Alterations in the miR-31-5p and IGF2BP1 levels in MDA-MB-231 (C) or BT-549 (D) cells following miR-31-5p overexpression. The IGF2BP1 protein level in the cells following the overexpression and knockdown of miR-27b-3p (E)/miR-31-5p (F) was examined using western blotting. Silencing or overexpression circRRM2, the expression of IGF2BP1 mRNA (G, H) and protein (I) was assessed by qRT-PCR and WB, respectively. Scratch wound healing assay showed the migration ability of IGF2BP1 silencing in BT-549 (J) or MDA-MB-231 (K) cells. The rescue effect of circRRM2 overexpression on IGF2BP1 knockdown in BT-549 (L) or MDA-MB-231 (M) cells was investigated. **P < 0.01, ***P < 0.001