Fig. 5
Analysis of the influence of ICL overexpression on growth of subcutaneous tumors and survival of tumor-bearing mice. (A) Tumor growth curve. The x-coordinate represents the injection time (weeks) and the y-coordinate represents the tumor volume (mm3). The number of animals in each group was six (n = 6). (B) Survival time of animals (n = 12). (C)The microvessel density in subcutaneous tumors was calculated. The immunostaining was visualized by fluorescence microscopy at 200× magnification. (D) The positive Ki67 assay. To analyze the Ki67 positive rate, the labeling index was performed. Three random images from every cell slide were photographed in triplicate. The labeling index was calculated by dividing the number of Ki67 positive cell nuclei by the total number of cells and multiplying by 100. (E) Detection of ICL and hsa-miR19-3p levels in subcutaneous tumor tissues by using RT-qPCR. (F) Detection of phosphorylation of NF-κB-p65 and protein expression of NKRF in subcutaneous tumor tissues by western blotting. Left, analysis of optical density. right: X-image of the target bands. Data are expressed as the means ± SD. ** p < 0.01, *p < 0.05