Fig. 6

MiR-3678-3p was targeted by SATB2-AS1. The miRNA targets of SATB2-AS1 and GRIM-19 were predicted by LncBase Predicted v.2 (http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex) and Targetscan (https://www.kegg.jp/), respectively. A: Venn diagram analysis was utilized to verify the common miRNA targets between SATB2-AS1 and GRIM-19. Six miRNAs (including has-miR-1184, has-miR-1205, has-miR-6501-3p, has-miR-17-3p, has-miR-3678-3p, has-miR-4713-3p) were common targets to SATB2-AS1 and GRIM-19. B. RT-PCR was conducted for detecting including has-miR-1184, has-miR-1205, has-miR-6501-3p, has-miR-17-3p, has-miR-3678-3p, has-miR-4713-3p in Huh7 cells transfected with vector or SATB2-AS1. C: The binding sites between miR-3678-3p and SATB2-AS1, miR-3678-3p, and GRIM-19. D, E: SATB2-AS1-WT/MUT and GRIM-19 3′UTR-WT/MUT vectors, along with miR-3678-3p mimics or NC mimics were transfected into Huh7 cells. Dual-luciferase reporter assay was performed. E. Huh7 cells were transfected with miR-3678-3p mimics, and an RIP assay was conducted. The enrichment levels of SATB2-AS1, GRIM-19, and miR-3678-3p in the lysates were detected by RT-PCR. F: FISH unveiled colocalization between miR-3678-3p (green) and SATB2-AS1 (red) in Huh7 cells. SATB2-AS1 probes and miR-3678-3p probes were labeled with Alexa Fluor 555 and Alexa Fluor 488, respectively. DAPI was employed for nuclei staining; Scale bar = 10 μm. ns indicates P > 0.05, *** indicates P < 0.001. N = 3