Fig. 3
From: FGF-trapping hampers cancer stem-like cells in uveal melanoma
Treatment with NSC12 reduces the self-renewal potential of UM cells. A 92.1, Mel270 and Mel285 cells were treated with increasing doses of NSC12 for 24 h. Then, ALDHbr cells were measured by cytofluorimetric analysis. Data are the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control, ANOVA. B Representative flow cytometry dot plots of control or 7 µM NSC12 treated UM cells. The blue gate refers to the positive/negative cell populations as identified in the presence of DEAB inhibitor (see Additional file 1: Fig. S2). C 92.1, Mel270 and Mel285 cells were treated with increasing doses of NSC12 for 24 h. Then 3000 viable cells were resuspended in melanospheres culture medium and plated. After 7 days, melanospheres were counted and photographed. Data are the mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001 vs control, ANOVA. D Representative images of melanospheres obtained from control or 7 µM NSC12 pre-treated UM cells. Scale bar: 100 µm