Fig. 2

The effect of S1PR2 on EGF-induced migration of OSCC cells was determined using the scratch wound assay. (A) Representative images captured using phase-contrast microscopy. (B) Scratch wound cultures were performed for 24 h, and EGF stimulated migration of H400 and H413 cell lines. This effect of inducing migration significantly decreased when S1PR2 was inhibited, but this did not increase when cells received both the EGF and S1PR2 agonist. H357 cells showed a similar trend to the other two cell lines, however a statistically significant difference in percentage gap closure was only identified between the control and group treated with both EGF and the S1PR2 agonist. (Scale bar shown represents 200 μm; Three biological replicates were performed with N = 4 for each experiment; ANOVA followed by post-hoc Tukey test, * = p-value < 0.05 and ** = p-value < 0.01, data presented as mean ± 1 SD.)