Fig. 5

CAFs-derived ROCK1 mediated the process of EMT to promote the ability of movement, migration, invasion of LSCC via activation of ROCK1/ JAK2 axis. (A) The expressions of ROCK1, ROCK2, p-JAK2, JAK2, p-STAT3, STAT3, p-ERK1/2 and ERK1/2 were detected by Western Blot in Hep2/AG490 + CAFs cells, levels of p-JAK2, p-STAT3, p-ERK1/2 were markedly decreased, while levels of ROCK1, ROCK2, JAK2, STAT3 and ERK1/2 were not changed. (B) Protein ratio in Hep2/AG490 + CAFs cells (**P < 0.01). (C) Hep2/parental + CAFs or Hep2/AG490 + CAFs on cell mobility as assessed via wound healing assay. Hep2/AG490 + CAFs cells decreased the mobility. (D) Wound size in Hep2/parental + CAFs or Hep2/AG490 + CAFs cells (*P < 0.05). (E) Hep2/parental + CAFs or Hep2/AG490 + CAFs cells on cell migration and invasion as measured via trans-well assay. Hep2/AG490 + CAFs cells inhibited the ability of migration and invasion. (F) Cell number in every field in Hep2/parental + CAFs or Hep2/AG490 + CAFs cells (*P < 0.05). (G) The expressions of E-cadherin and N-cadherin, Slug and Vimentin were detected by Western Blot in Hep2/AG490 + CAFs cells, level of E-cadherin was markedly increased, while levels of N-cadherin, Slug and Vimentin were decreased. (H) Protein ratio in Hep2/AG490 + CAFs cells (*P < 0.05, **P < 0.01)