Fig. 1

Local X-ray irradiation changed the metastatic nodule numbers in lung of 4T1 tumor-bearing BALB/c mice. a Schematic representation of tumor development, and treatment timeline. b The tumor volume, c tumor weight of tumor-bearing mice in different groups was shown (*P < 0.05). d Survival time of tumor-bearing mice in different groups. e Number of lung metastatic nodules were counted based on H&E-stained slides (*P < 0.05). The experiment was repeated three times with 6 mice in each group. f representative images of metastatic nodules. Images are shown at 4 x magnification. Tumor sections from all 18 mice/group were examined. g Vimentin or E-Cadherin expression of tumors from IR or non-IR mice were confirmed by western blotting