Fig. 6

X-ray irradiation enhances G-CSF, CXCL1 and GM-CSF production by 4T1 tumor cells. a The cytokine/chemokine profile in the 4T1-exo or ir/4T1-exo were examined by mouse cytokine array. Pixel density in each spot of the array was determined using ImageJ. b The content of G-CSF, CXCL1 and GM-CSF in the exosomes was measured by ELISA. Compared with 4T1-exo, *p < 0.05, **p < 0.01. c and d The level of G-CSF, CXCL1, GM-CSF and CCL2 in lung tissues of 4T1 tumor-bearing mice before or after radiotherapy (n = 3 per group) was detected with the method of ELISA. Compared with normal and untreated mice, **p < 0.01. e CCL2 level in lung extracts of normal BALB/c mice after receiving exosomes injection. Compared with injection of PBS or 4T1-exo, **p < 0.01 or # p < 0.05, respectively. f CCL2 level in cell culture supernatant or exosomes derived from 4T1 or irradiated-4T1 cells. g The level of CCL2 in mouse lung tissue extracts was positively correlated with the number of M-MDSCs.