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Fig. 2 | Cancer Cell International

Fig. 2

From: Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis

Fig. 2

Enhanced FAO is required for BM-MSCs education by LNM-GC-sEV. A CPT1A was detected by western blotting in BM-MSCs treated with etomoxir and then incubated with GC-sEV. B–D The activity of CPT1, β-oxidation rate and ATP level of the above treated BM-MSCs were measured. E–K In vitro analysis of the effects of etomoxir on the education of BM-MSCs by GC-sEV. E Immunofluorescence staining for α-SMA. F, G Tubule formation assay. H–K Migration and invasion assay. L–N In vivo analysis of the effect of etomoxir on the tumor-promoting capacity of BM-MSCs induced by GC-sEV. L The harvested popliteal LNs. M Weight of the popliteal LNs. N IHC analysis of pan-cytokeratin (AE1/AE3) expression in popliteal LNs. **P < 0.01; ***P < 0.001; ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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