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Fig. 3 | Cancer Cell International

Fig. 3

From: Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis

Fig. 3

Knockdown of CD44 abrogates LNM-GC-sEV prompting FAO metabolic reprogramming of BM-MSCs. A Schematic illustration of BM-MSCs treated with sEV from NC and si-CD44 transfected HGC-27L respectively. B Western blotting analysis of CPT1A expression in the above sEV treated BM-MSCs. C–E Comparison of CPT1 activity, β-oxidation rate and ATP level in BM-MSCs between NC-sEV group and si-CD44-sEV group. F–L Comparison of BM-MSCs education by NC-sEV and si-CD44-sEV. F Immunofluorescence staining for α-SMA. G, H Tubule formation analysis. I–L Migration and invasion assays. M Representative images of popliteal LNs. N Weight of popliteal LNs. O Representative images of pan-cytokeratin (AE1/AE3) in popliteal LNs detection by IHC staining. *P < 0.05; ***P < 0.001; ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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