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Fig. 4 | Cancer Cell International

Fig. 4

From: Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis

Fig. 4

CD44 enrichment enables primary GC-sEV to acquire similar regulatory effects of LNM-GC-sEV on BM-MSCs. A, B Western blotting analysis of CD44 in AGS and their sEV after CD44 overexpression. C Western blotting analysis of CPT1A expression in BM-MSCs treated with vector-sEV and CD44-sEV. D–F Measurement of CPT1 activity, β-oxidation rate and ATP level in BM-MSCs treated with vector-sEV and CD44-sEV. G–M Comparison of BM-MSCs education by vector-sEV and CD44-sEV. G Immunofluorescence staining for α-SMA. H, I Tubule formation assay. J–M Transwell migration and invasion assays. N Representative images of popliteal LNs. O Popliteal LNs weight. P IHC staining of pan-cytokeratin (AE1/AE3) in popliteal LNs. **P < 0.01; ***P < 0.001; ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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