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Fig. 6 | Cancer Cell International

Fig. 6

From: Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis

Fig. 6

IL-8 and STC1 are critical for oncogenic roles of the educated BM-MSCs. A GSEA plots of indicated signature genes enriched in BM-MSCs incubated with si-CD44-sEV. B The Heatmap of the selected down-regulated genes related to secreted factors in si-CD44-sEV group compared with NC-sEV group. C–H qPCR of IL-8 and STC1 in BM-MSCs under indicated treatment. I-L Migration and invasion assays. M Tubule formation assay. N, O GC cells were treated with CM collected from BM-MSCs incubation with AGS-sEV and HGC-27L-sEV; Western blot analysis of CD44 levels in GC cells and their sEV. P CM from BM-MSCs educated by HGC-27L-sEV was incubated with IL-8 neutralizing antibody and STC1 antibody, respectively, and then were used to treat HGC-27. Western blotting analysis of CD44 and CPT1A in HGC-27. ***P < 0.001; ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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