Fig. 8

Expression validation of CLEC1B in HCC cells. (A, B) RT-qPCR (A) and western blot (B) detection of CLEC1B expression levels in HCC and normal hepatic liver cells. (C) Representative images of CLEC1B immunohistochemical staining of paired HCC and adjacent normal tissues. (D) Histogram shows the quantification data of immunohistochemical staining. (E) Protein expression of CLEC1B in SMMC-7721 plvx-neo and SMMC-7721 CLEC1B-neo cells. (F) Cell viability of SMMC-7721 plvx-neo and SMMC-7721 CLEC1B-neo upon sorafenib administration. (G) Flow cytometric analysis of apoptosis distribution after sorafenib treatment in SMMC-7721 cells, and the quantification data were shown on the right. **P-value < 0.01, ***P-value < 0.001