Fig. 5

Regulatory effects of PZH on ferroptosis related pathways in the liver tissues of DEN induced HCC rats. A Kaplan–Meier survival curve analysis of SLC7A11 expression profile on prognosis of HCC patients. B Effect of PZH on Fe²⁺ content in the liver tissues of different groups. C Regulatory effects of PZH on serum GSH and GSSG contents of different groups. D, G Regulatory effects of PZH on liver iron deposition in different groups. Blue color is iron deposition. E, H Immunohistochemistry staining observed the effect of PZH on GPX4 expression in the liver tissues of different groups, orange color is positive expression. F, I Western blot assay detected the effects of PZH on SLC7A11 protein expression in the liver tissues of different groups. Data are expressed as mean ± standard deviation. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with the normal group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with the DEN model group