Fig. 1

Baseline expression level of inhibitory receptors in T cells of patients with EOC and healthy controls. The baseline percentage of TIM3+ (A), PD1+ (B), and A2aR+ (C) cells are significantly higher in EOC patient-derived T cells post thawing. The frequency of T_tex cells is significantly higher in patients’ samples compared to normal controls (D). However, the frequency of T_pex cells is not significant different between patients and healthy samples (D). Representative overlay histogram plots representing expression level of TIM3 (A), PD1 (B), and A2aR (C) in EOC patient-derived T cells sample (Red color) vs. unstained sample (Blue color). Representative overlay dot plot (D) representing the frequency of terminally exhausted and progenitor exhausted T cells in an EOC patient sample (Red color) vs. unstained sample (Blue color). Data from healthy donors (n = 30) and EOC patients (n = 30) were analyzed using Welch’s t-test (A and B) Mann-Whitney U test (C and D). *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as mean ± SD.