Fig. 2

MFI2 is involved in the migration and invasive ability of OSCC cells. A SAS, SCC4 and KOSC3 cells were transfected with control siRNA and MFI2-specific siRNA. The protein expression of MFI2 was analyzed by western blot. β-Actin was used as the loading control. B Cell proliferation assay (CCK-8) demonstrated that MFI2-knockdown inhibited the proliferation of SAS, SCC4 and KOSC3 cells. Data are presented as the mean of three experiments. The transfected cells were subjected to migration (C) and invasion (D) assays. Representative microphotographs of the filters obtained from the migration and invasion assays. Original magnification: ×100 (left). Quantitative analysis of the migration and invasion assays (right). Data are presented as the mean values obtained from three independent experiments