Fig. 3

EGF positively correlates with MFI2 in OSCC. A Transcript expression levels of MFI2 from OSCC patient subgroups stratified by high and low MFI2 expression. B Correlation analysis between the transcript T/N fold of EGF and MFI2 from OSCC patients. C Gene expression of EGF in control and MFI2-specific siRNA-transfected SCC4 and KOSC3 cells was analyzed by qPCR. β-Actin was used as a normalized control. These data are representative of three independent experiments (*p < 0.05, **p < 0.01). D Western blot analyses of EGF and MFI2 in conditioned medium (CM) and cell lysate (CL), respectively (left). Representative SDS-PAGE stained by Coomassie blue showing total proteins in CM (right)