Fig. 4

Phosphorylation of FAK is decreased in MFI2-knockdown SCC4, KOSC3 and SAS cells. A Control siRNA and MFI2-specific siRNA were transfected into SCC4, KOSC3 and SAS cells for 24 h and then incubated with serum-free medium for 24 h. Western blot analysis of the expression of MFI2, pFAK, FAK, pSrc, Src, pAkt, and Akt. β-Actin was used as a loading control. B Quantitative levels of pFAK, pSrc and pAkt in (A). The results are representative of three independent experiments (*p < 0.05, **p < 0.01). C Transfected cells were treated with EGF for 0.5, 8 or 24 h. Western blot of the expression of MFI2, pFAK, FAK, pSrc, Src, pAkt, and Akt. β-Actin was used as a loading control. D Quantitative level of pFAK in (C). The results are representative of three independent experiments (*p < 0.05, **p < 0.01)