Fig. 2

Effect of moricin peptide treatment on cell viability in MDA-MB-231 cells (A–D) Determination of toxic doses of moricin in MDA-MB-231 cells. Cells were cultured and incubated for 24 h without moricin (untreated) and with different concentrations of moricin (0–100 100 µg/ml). A Represents the morphology of moricin treated MDA-MB 231 cells with respect to untreated cells. Images were taken with Zeiss Axio-Observer, Germany at 20X magnification at scale bar 200 µm (B, C) represents the percent of cell viability and toxicity with respect to untreated cells as measured by MTT, LDH, and trypan blue assay respectively. Bar graphs are expressed as mean ± S.E of three independent experiments. Statistical analysis was performed using one way ANOVA test followed by Dunnett’s post hoc comparison test *p < 0.05, **p < 0.01, and ***p < 0.001 is significant as compared to the untreated cells