Fig. 3
Effect of moricin peptide treatment on intracellular ROS in MDA-MB-231 cells A Microscopy analysis were performed in MDA-MB-231 cells to measure intracellular ROS, cells were exposed to 6.25 µg/ml and 12.5 µg/ml concentrations of moricin to measure ROS with H2-DCFDA. Images were taken with florescent microscopy (Zeiss Microsystems, GmBH, Germany) at 20X magnification B Represents the mean fluorescence of DCFDA at 30 min. The fluorescence intensity of DCFDA was determined at 515/490 nm. C Represents the flow cytometric analysis to measure ROS with H2-DCFDA staining. Results are the mean ± S.E from three independent experiments and statistical analysis was determined one-way ANOVA test followed by Dunnett’s post hoc comparison test **p < 0.01, and ***p < 0.001 vs untreated cells