Fig. 4

Effect of moricin peptide treatment on Glutathione and TBARS level in MDA-MB-231 cells A Microscopy analysis were performed in MDA-MB 231 cells to measure GSH in cells treated with 6.25 µg/ml and 12.5 µg/ml concentrations of moricin using Monochlorobimane (mBCI) staining. Images were taken with florescent microscopy (Zeiss Microsystems, GmBH, Germany) at 20X magnification at scale bar 50 µm B Represents the level of GSH (nmol/µg) in MDA-MB-231 cells treated with 6.25 µg/ml and 12.5 µg/ml concentrations of moricin. C Represents the level of TBARS (µM) in MDA-MB-231 cells treated with 6.25 µg/ml and 12.5 µg/ml concentrations of moricin. Results are the mean ± S.E from three independent experiments and statistical analysis was determined one-way ANOVA test followed by Dunnett’s post hoc comparison test *p < 0.05, and ***p < 0.001 vs untreated cells