Fig. 7

Moricin exposure leads to Notch1/NF-ƙB mediated activation of programme cell death A Western blot analysis of NF-ƙB, Notch1, C p53, Bcl-2 and E Bax, caspase3 and 9 protein expression respectively in moricin exposed MDA-MB-231 cells treated with 6.25 µg/ml and 12.5 µg/ml moricin peptide. B Densitometric analysis of NF-ƙB and Notch1 protein bands (fold change) as compared to untreated cells, after normalization with β-actin. D Densitometric analysis of p53 and Bcl-2 protein bands (fold change) as compared to untreated cells, after normalization with β-actin. F Densitometric analysis of Bax, caspase3 and 9 protein bands (fold change) as compared to untreated cells, after normalization with β-actin. Densitometric analysis of bands was performed using Image studio, lite version 5.2 software (LI-COR) and values are expressed as fold change after normalization with β-actin. The bar graphs are presented as mean ± S.E of three independent experiments and statistical analysis was determined by two-way ANOVA test followed by Dunnett’s post hoc comparison test **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs untreated cells, ####p < 0.0001 vs untreated cells and $$$$p < 0.0001 vs untreated cells