Fig. 4

HOPX inhibits proliferation, migration, and invasion and induces apoptosis and cycle arrest in SKCM cell lines. (A) The overexpression of HOPX in A375 cells was confirmed by RT‒qPCR and Western blotting (B). (C) A CCK-8 assay was used to detect the proliferation of A375 cells. (D) A wound healing assay was used to detect the migration of A375 cells. (E-H) Transwell assays were used to detect the migration/invasion of A375 cells. (I-J) The apoptosis and cell cycle (K-L) of A375 cells were detected by flow cytometry