Fig. 5

Transcriptome sequencing analysis of HOPX in the SKCM cell line A375. (A) Volcano plots were drawn for all genes analyzed for differential expression (|Log₂FC| >1& adjusted P < 0.05). (B) A heatmap was drawn with samples as horizontal coordinates and differentially expressed genes as vertical coordinates. (C-D) Construction of the GO/KEGG enrichment circle diagram. The first circle (from outside to inside) shows the most GO entry/KEGG pathway (minimum P value), and the number outside the circle indicates the number of enriched genes, with different colors indicating different classifications; the second circle represents the number of genes annotated to the GO entry/KEGG pathway, and the third circle shows the statistics of the number of differentially upregulated and downregulated genes in the GO/KEGG pathway; the fourth circle indicates the percentage of enrichment factors. (E-F) Construction of GSEA-GO/KEGG enrichment bar graph. The top 30 gene sets with the smallest P and FDR values from GSEA-GO/KEGG analysis are plotted, the horizontal coordinates are the NES values of the gene sets, and the colors represent the P values