Table 1 RT-PCR primer sequences for PRKCG, Hif-1 alpha, VEGF, SOCS3, AKT and β actin
Genes | Primer sequences (5ʹ-3ʹ) | Tm (oC) | Ta (oC) | Product size | References |
|---|---|---|---|---|---|
PRKCG | F: CCTTCTGCGACCACTGT R: GCTGCAGTTGTCAGCAT | 54.8 53.4 | 58 | 557 bp | [38] |
Hif 1 alpha | F: CATCAGCTATTTGCGTGTGAGGA R: AGCAATTCATCTGTGCTTTCATGTC | 57.4 56.2 | 58 | 83 bp | [39] |
VEGF | F:CCTGGTGGACATCTTCCAGGAGTAC R: GAAGCTCATCTCTCCTATGTGCTGG | 62 60.8 | 61 | 196 bp | [39] |
SOCS 3 | F: ATTTCGCTTCGGGACTAGC R: AACTTGCTGTGGGTGACCAT | 55 57.1 | 58 | 126 bp | [40] |
AKT | F: TAGGCATCCCTTCCTTACAGC R: CACTGTCCCATCCGGCTTCA | 56.4 59.7 | 55 | 114 bp | [41] |
Beta actin | F:GGGGTGTTGAAGGTCTCAAA R:TGTCACCAACTGGGACGATA | 55 55.7 | 57 | 165 bp | [42] |