Table 2 ARMS PCR primer sequences for PRKCG SNP (rs386134171, G/A)
SNP | Primer sequences (5ʹ-3ʹ) | Tm(oC) | Ta(oC) | Product size |
|---|---|---|---|---|
rs386134171 (G/A) | Forward inner primer (G allele): 160 CTTCAGCTTCCTCATGGTTCTAGGAACAG 188 | 68 °C | 60 °C | 175 bp |
Reverse inner primer (A allele): 214 CAGGAATCCAACCTTCCCAAAACTTCT 188 | 68 °C | 193 bp | ||
Forward outer primer (5ʹ—3ʹ): 22 TCTGGCTCTTTCTTTCTCCTTTCCACAG 49 | 68 °C | 312 bp | ||
Reverse outer primer (5ʹ—3ʹ): 333 ACGGCCCAACCTAACCATATTTGAAGAT 306 | 68 °C |