Table 7 MutPred2 conservation scores and the underlying molecular mechanisms disrupted because of alteration in amino acid residues

C52S

0.960

Altered Transmembrane protein;

Altered Metal binding;

Gain of Disulfide linkage at C49

0.29

0.28

0.12

3.7e-04

0.02

0.04

C114Y

0.955

Altered Metal binding;

Altered Transmembrane protein;

Gain of Sulfation at C114

0.56

0.12

0.02

2.9e-03

0.03

0.04

D115Y

0.932

Altered Metal binding;

Altered Transmembrane protein;

Gain of Sulfation at D115

0.56

0.09

0.01

2.9e-03

0.05

0.04

G128R

0.869

Altered Metal binding

0.29

0.02

C131Y

0.901

Altered Metal binding;

Gain of Pyrrolidone carboxylic acid at Q127

0.41

0.04

6.1e-03

0.05

Gain of Strand

0.26

0.04

V138M

0.431

-

-

-

C150R

0.970

Altered Metal binding;

Altered Transmembrane protein;

Loss of Disulfide linkage at C150

0.55

0.11

0.22

3.0e-03

0.04

0.01

Gain of Intrinsic disorder;

Altered Disordered interface

0.35

0.22

0.15

0.02

0.01

0.05

G159R

0.741

Altered Transmembrane Protein

0.13

0.02

Gain of Helix

0.32

2.2e-0.3

G360S

0.908

Loss of Acetylation at K364;

Altered DNA binding;

Loss of Methylation at K364;

Loss of Catalytic site at K359

0.24

0.20

0.15

0.14

0.02

0.02

0.02

0.03

L403P

0.971

Gain of Allosteric site at K400

0.23

0.02

Gain of Intrinsic disorder

0.35

0.02

L468P

0.932

Altered Metal binding;

Gain of Allosteric site at H472;

Altered Transmembrane protein;

Altered DNA binding

0.31

0.26

0.14

0.14

2.2e-03

9.1e-03

0.02

0.05

Altered Ordered interface;

0.28

0.04

D480Y

0.946

Altered Metal binding;

Loss of Allosteric site at R479;

Altered DNA binding;

Altered Transmembrane protein;

Loss of Catalytic site at D484;

Gain of Sulfation at D480

0.77

0.31

0.23

0.15

0.14

0.01

5.4e-05

3.5e-03

0.01

0.01

0.03

0.04

Altered Ordered interface;

Altered Disordered interface;

0.32

0.28

1.8e-03

0.04

Y521C

0.945

Altered Metal binding;

Gain of Catalytic site at C516;

Loss of Allosteric site at Y521;

Altered Transmembrane protein;

Gain of Disulfide linkage at C516;

Loss of Sulfation at Y521;

0.81

0.37

0.33

0.30

0.16

0.04

5.7e-04

2.8e-04

1.9e-03

1.3e-04

0.03

0.01

Altered Ordered interface;

Altered Disordered interface;

Gain of Relative solvent accessibility

0.44

0.29

0.26

5.7e-04

0.02

0.03

R597H

0.771

Altered DNA binding;

Loss of Allosteric site at R597;

Loss of Acetylation at K592;

Loss of Amidation at P594

0.37

0.37

0.25

0.01

6.8e-04

9.2e-04

0.01

1.2e-03

F643L

0.865

Loss of Acetylation at K645

0.40

1.3e-03

Altered Ordered interface;

Gain of Helix

0.29

0.27

0.03

0.04