Fig. 14

Exploration and Validation of the Interrelationships among the Three Model Genes. (A–C) Pearson correlation analysis revealed significant positive regulatory associations between CTSW and CD3D (A), CD3D and CD48 (B), as well as CD48 and CTSW (C) among the three model genes. (D–F) qRT-PCR validation of the designed siRNAs targeting CTSW (D), CD3D (E), and CD48 (F) in HEC-1A cell line, showing the downregulated expression levels of the target genes in the gene knockdown cells compared to the negative control group. (G–I) Further qRT-PCR validation of the designed siRNAs targeting CTSW (G), CD3D (H), and CD48 (I) in ISHI cell line, demonstrating the downregulated expression levels of the target genes in the gene knockdown cells compared to the negative control group. (J–L) The expression differences between SiCTSW (J), SiCD3D (K), and SiCD48 (L) and the negative control for the other two target genes. (M–O) Further qRT-PCR analysis in ISHI cell line exploring the expression differences of SiCTSW (M), SiCD3D (N), and SiCD48 (O) compared to the negative control for the remaining two target genes. Among them, ns: no significant statistical difference, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. qRT-PCR data are means ± SD, with n = 3