Fig. 1
From: CircGNB1 facilitates the malignant phenotype of GSCs by regulating miR-515-5p/miR-582-3p-XPR1 axis
CircGNB1 is up regulated in glioma tissues and correlated with poor prognosis. a CircRNA sequences between glioma tissues and normal brain tissues in GSE146463. b Volcano plots of circRNAs showed different expression between glioma tissues and NBTs. c Schematic illustration of genomic location and back splicing of circGNB1 with the junction sites via Sanger sequencing. d, e Agarose gel electrophoresis analysis confirmed the presence of circGNB1 in GSCs. f, g RNase treatment was used to evaluate the stability of circGNB1 and GNB1 mRNA in GSCs. h FISH assays confirmed the cellular localization of circGNB1 in GSCs. i PCR measured the relative expression of circGNB1 in NBTs (n = 10), grade II (n = 20), grade III (n = 25), and grade IV (n = 25) glioma tissues, and the results showed that circGNB1 was positively associated with the degree of malignancy. j Survival curve of glioma patients showed that the survival time was negatively associated with the expression of circGNB1. All data were expressed as mean ± SD, and each experiment was performed in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001