Fig. 4

The 3′UTR of XPR1 mRNA is the common direct target of miR-515-5p and miR-582-3p. a Identification and intersection of a mRNA potentially targeted with both miR-515-5p and miR-582-3p based on microRNA, miRWalk, Starbase, and TargetScan databases. b–e qPCR showed the relative gene expression in GSCs after miR-515-5p/miR-582-3p inhibitor (or mimic) treatment. f–i Western blotting showed the expression of XPR1 following miR-515-5p/miR-582-3p inhibitor (or mimic) treatment. j, k Schematic diagram of the putative miR-515-5p and miR-582-3p binding site in XPR1. l–o The luciferase reporter assays showed that miR-515-5p/miR-582-3p inhibitor (or mimic) affected the 3′UTR region of XPR1 mRNA in GSCs. Data represent mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001