Fig. 2

Cytokines production in CTH cultures. Cytokines TGF-β (a), IL-10 (b), IL-1β (c), and IL-6 (d) were measured in supernatants of CTH cultures by ELISA kits. Remarkably, TGF-β concentration was enhanced along with the PGCCs count in the CTH-DB culture. IL-10 concentration was reduced in the CTH-BL culture with the presence of a high PGCCs count. The higher IL-6 expression was detected in CTH-DB culture. Data are represented as mean ± SD of two independent experiments. Blue and orange curves represent the cytokine concentration (pg/ml) (Left Y-axis) and PGCCs count/well (Right Y-axis), respectively. The X-axis represents the days post-infection