Fig. 7

Exosome-derived circKIF20B transfers the malignant state and gefitinib resistance of NSCLC in vitro. A The identification of exosomes extracted from Beas-2B, PC9, and PC9GR by electron microscopy. B The expression of circKIF20B, miR-615-3p, and MEF2A in exosomes of Beas-2B, PC9, and PC9GR, as detected by qRT-PCR. C The DiO-labeled exosomes extracted from the circKIF20B-OE PC9 cells were co-cultured with PC9 for 24 h. D Dio-labeled overexpressed circKIF20B PC9 cell-derived exosomes were endocytosed by the recipient PC9 cells. E The expression of circKIF20B, miR-615-3p, and MEF2A in the PC9 cells co-cultured with exosomes from the circKIF20B-OE cells, as measured by qRT-PCR. F and G EdU assay of PC9 cells co-cultured with the exosomes (without gefitinib or with 0.05µM gefitinib). H Cell cycle assay of PC9 cells co-cultured with exosomes from circKIF20B-OE PC9 cells. I Gefitinib IC50 values of PC9 cells co-cultured with exosomes from circKIF20B-OE PC9 cells. J and K The OCR analysis of PC9 cells co-cultured with the exosomes from circKIF20B-OE PC9 cells