Fig. 5

Hsa_circ_000200 acts as the sponge of miR-4659a/b-3p. A Level of hsa_circ_000200 detected by qRT-PCR after RIP for Ago2. B Validation of the levels of miRNAs that may bind to hsa_circ_000200 after overexpression of hsa_circ_000200 in HEK-293T cells. C Validation of the levels of miRNAs that may bind to hsa_circ_000200 after overexpression of hsa_circ_000200 in MKN-45 cells. D The potential binding site of miR-4659a/b-3p in hsa_circ_000200 is predicted by bioinformatic software. E The binding of hsa_circ_000200 and miR-4659a/b-3p is demonstrated by dual-luciferase reporter assay in MKN-45 cells. F Expression of miR-4659a/b-3p in GES-1, HGC-27, AGS and MKN-45. G. Assessment of the proliferation in MKN-45 and HGC-27 cells transfected with control or miR-4659a/b-3p mimics by CCK8 assay (G left and middle); assessment of the proliferation in AGS cells transfected with control or miR-4659a/b-3p inhibitor by CCK8 assay (G right). H. The number of cell clones in MKN-45 and HGC-27 after miR-4659a/b-3p mimics transfection (H above); the number of cell clones in AGS after miR-4659a/b-3p inhibitor transfection (H below). I The results of migration assays in MKN-45 and HGC-27 after miR-4659a/b-3p mimics transfection (I above); the results of migration assays in AGS after miR-4659a/b-3p inhibitor transfection (I below). *P < 0.05, **P < 0.01, ***P < 0.001