Fig. 6

Hsa_circ_000200 promotes metastasis and proliferation of GC through regulating miR-4659a/b-3p/HBEGF axis. A Assessment of the proliferation in AGS cells transfected with hsa_circ_000200 plasmid and miR-4659a/b-3p mimics by CCK8 assay. B The results of migration assays in AGS after hsa_circ_000200 plasmid and miR-4659a/b-3p mimics transfection. C The binding of miR-4659a/b-3p and HBEGF is proved by dual-luciferase reporter assay in HEK-293T and HGC-27 cells. D The expression of HBEGF in 27 paired GC tissues and paracancer tissues. E. In the GC tissues, the level of hsa_circ_000200 and HBEGF were positively correlated by using Pearson correlation analysis. F The western blot results of HBEGF after hsa_circ_000200 ASO/plasmid transfection. G The results of migration assays in AGS after hsa_circ_000200 plasmid and si-HBEGF transfection. H, I The western blot results after hsa_circ_000200 plasmid and si-HBEGF transfection. *P < 0.05, **P < 0.01, ***P < 0.001