Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Tumor-derived small extracellular vesicles promote breast cancer progression by upregulating PD-L1 expression in macrophages

Fig. 3

BC cells promoting the polarization of M2 macrophages and PD-L1 expression in vitro. (A) Co-culture schematic diagram between macrophages derived from CD14 + monocytes and BC cells MDA-MB-231. (B) Representative optical microscope representations of CD14 + monocytes, induced M0 macrophages, and macrophages after 48 h of co-culture. (C) The expression of M2 markers (CD206, ARG1 and IL-10) detected by RT-qPCR in CD14 + monocytes derived M0 after co-culture. (D) The proportion of CD163 + CD68 + macrophages after co-culture analyzed by flow cytometry. (E) The PD-L1 expression of CD14 + monocytes derived M0 after co-culture detected by IF. (F) Co-culture schematic diagram between THP-1-derived M0 and MDA-MB-231 cells. (G) Representative optical microscope pictures of THP-1 cells induced M0, and macrophages after 48 h of co-culture. (H) The expression of M2 markers (CD206, ARG1 and IL-10) detected by RT-qPCR. (I) The proportion of CD163 + CD68 + macrophages after co-culture which was analyzed by flow cytometry. (J) The PD-L1 expression detected after co-culture by IF.

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us