Fig. 4

Culturing patient-derived cells affect the expression of chemokine genes. Differential expression (DE) analysis from RNA sequencing transcriptome profile revealed significant changes in the expression of genes related to chemokines in all culturing conditions compared to both fresh tissue sample and freshly isolated single cells. All molecules in this family, except CXCL5, were downregulated. The genes that passed the filter criteria had a p < 0.05. P values in blue represent the comparison with tissue, while the p values in red represent the comparisons with freshly isolated single cells. The mean is presented as a red box and the median as a black line