Fig. 5

Lnc-LRRTM4 enhances LRRTM4 transcription to promote CRC progression. (A) The effects of LRRTM4 knockdown on cell cycle in HCT116 and SW480 cells and the effects of LRRTM4 overexpression on cell cycle in LoVo cells. (B) The effects of LRRTM4 knockdown on apoptosis in HCT116 and SW480 cells and the effects of LRRTM4 overexpression on apoptosis in LoVo cells. (C) The effects of LRRTM4 knockdown on cell cycle proteins and apoptosis proteins in HCT116 and SW480 cells and the effects of LRRTM4 overexpression on cell cycle proteins and apoptosis proteins in LoVo cells. (D) The effects of LRRTM4 knockdown on EMT proteins in HCT116 and SW480 cells and the effects of LRRTM4 overexpression on EMT proteins in LoVo cells. (E) Probe of lnc-LRRTM4 and negative control LacZ were used for ChIRP, followed by qPCR of LRRTM4 promoter in HCT116 and SW480 cells. (F) Luciferase plasmid of LRRTM4 promoter was transfected into HCT116 and SW480 cells with or without lnc-LRRTM4 siRNA, followed by detection of luciferase activity. Data are the means ± SD (n = 3 independent experiments), * p < 0.05, ** p < 0.01, *** p < 0.001