Fig. 4

Effect of moricin peptide treatment on structural damages of mitochondria and lysosomes and induced mitochondrial ROS generations in MDA-MB-231 cells. A Mitochondrial ROS was determined by microscopy with mitoSOX staining of moricin treated and untreated MDA-MB-231 cells treated with 6.25 µg/ml and 12.5 µg/ml moricin peptide. Images were taken with florescent microscopy (Zeiss Microsystems, GmBH, Germany) at ×20 magnification at scale bar 100 µm. B Represents level of mitochondrial ROS through relative mitoSOX florescence. C Fluorescent images of the MDA-MB-231 cells treated with 6.25 µg/ml and 12.5 µg/ml moricin peptide. Staining was done for the nucleus (blue), mitochondria (green) and lysosome (red) using Hoechst 33342 (10 µg/ml), Mitotracker Green FM (75 nM), and Lysotracker Red (100 nM). Cell shrinkage and mitochondrial disruption are seen for cells treated with moricin but not in the control cells. Images were taken with florescent microscopy (Zeiss Microsystems, GmBH, Germany) at ×20 magnification at scale bar 150 µm. Results are the mean ± S.E from three independent experiments and statistical analysis was determined one-way ANOVA test followed by Dunnett’s post hoc comparison test ****p < 0.0001 vs untreated cells