Fig. 6

MAL2 regulates β-catenin /c-Myc signaling. A Western blot analysis of MAL2, β-catenin and c-Myc expression in BC cells after silencing MAL2. B Immunoprecipitation was performed with MDA-MB-231 cell lysates using MAL2 or β-catenin antibodies, and the precipitants were detected by western blot with the indicated antibodies. C Double immunostaining of MAL2 (red) and β-catenin (green) in the MDA-MB-231 cells. Scale bar: 20 μm. D MDA-MB-231and MCF-7 cells infected with sh-NC and sh-MAL2 were treated with β-catenin inhibitor XAV-939 (20 µmol/L) for 24 h. E MDA-MB-231and MCF-7 cells infected with sh-NC and sh-MAL2 were treated with β-catenin agonist SKL2001 (20 µmol/L) for 24 h. The protein expression of β-catenin and c-Myc were evaluated by western blot assay. All data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001