Fig. 4

SQLE knockdown inhibited T24 and 5637 cell proliferation, migration, and viability. (A, B) The qRT-PCR assay detected shRNAs transfection efficiency in two BCa cell lines T24 and 5637; (C) Western blot assay detected the sh2 efficiency of transfection for protein level in two BCa cell lines T24 and 5637; (D, E) The CCK-8 assay was used to evaluate the proliferation of shSQLE-treated tumor cells and NC-treated tumor cells from 0 to 96 h; (F) Representative images of the colony-forming assay; (G, H) Immunofluorescence results showed that the expression of KI67 was decreased in BCa; (I, J) The wound-healing assay demonstrated that the capacity of migration in two BCa cell lines T24 and 5637 (K–M) The migration and invasion of T24 and 5637 cell lines were inhibited by SQLE knockdown as measured by a transwell assay. Data represent the mean ± standard deviation (SD) of three independent experiments. (The magnification under the microscope is shown as marked in the diagram; *p < 0.05, **p < 0.01, ***p < 0.001 versus the vector group, ns, not significant)