Fig. 7

SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + PFT β from 0 to 96 h; (D) Representative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)