Table 2 Details of antibodies
Antibodies | Company | Catalog number | Source | Dilutions |
|---|---|---|---|---|
SQLE | Abmart | TD12063 | Rabbit | WB:1:1000 IF/IHC:1:100 |
KI67 | Proteintech | 27309-1-AP | Rabbit | 1:200 |
Phospho-AKT | Proteintech | 66444-1-Ig | Mouse | WB:1:10000 IF/IHC:1:100 |
AKT | Proteintech | 60203-2-Ig | Mouse | 1:10000 |
Phospho-GSK-3β | Cell Signalling Technology | #9323 | Rabbit | 1:1000 |
GSK-3β | Cell Signalling Technology | #9832 | Mouse | 1:1000 |
PTEN | Proteintech | 60300-1-Ig | Mouse | WB:1:1000 IF:1:200 |
P53 | Abcam | ab26 | Mouse | WB/IF:1 µg/ml |
cyclin D1 | Proteintech | 26939-1-AP | Rabbit | WB:1:10000 IHC:1:500 |
Bcl-2 | Cell Signalling Technology | #15,071 | Mouse | 1:1000 |
Bax | Proteintech | 60267-1-Ig | Mouse | WB:1:5000 IHC:1:500 |
Cleaved-Caspase-3 | Cell Signalling Technology | #9664 | Rabbit | 1:1000 |
GAPDH | Proteintech | 60004-1-Ig | Mouse | 1:20000 |