Fig. 4

Analysis of EZH2 repression of let-7c and miR-99a expression. A. EZH2 was transfected into HepG2 and Hep3B cells. The expression of EZH2 was elevated in EZH2-expressing HepG2 and Hep3B cells as compared with cells transfected with the empty vector. let-7c and miR-99a expression was normalized against the control miRNA U48, as measured using qRT-PCR. B. The expression of let-7c and miR-99a was elevated in HepG2X and Hep3BX cells after knockdown of EZH2 by shRNAs. C. HepG2X and D Hep3BX cells handled with DZNep (10 µM) or DMSO (vehicle control) for 48 h. were executed to western blot analysis with anti-EZH2. The expression of let-7c and miR-99a was also determined in the same cells by qRT-PCR.