Table 1 The primer sequence of genes for PCR and QPCR
Gene | Sequence | |
|---|---|---|
PCR | ||
HBx forward | GTTAAGCTTATGGCTGCTAGGCTGTGCTGC | |
HBx reverse | AGACTCGAGCCGGGCAGAGGTGAAAAAGTTGC | |
EZH2 forward | GCAGTAAAAATGTGTCCTGCAAGAA | |
EZH2 reverse | TCAAGGGATTTCCATTTCTCTTTCGA | |
GAPDH forward | CATCCCTGCCTCTACTGGCG | |
GAPDH reverse | AGGCCATGTGGGCCATGAGG | |
QPCR | UPL probe | |
HBX forward | TTGGGGGAGGAGATTAGGTT | 75 |
HBX reverse | TGGTGAACAGACCAATTTATGC | |
EZH2 forward | GACTGGCGAAGAGCTGTTTT | 64 |
EZH2 reverse | TCTTTCGATGCCGACATACTT | |
HMGA2 forward | TCCCTCTAAAGCAGCTCAAAA | 34 |
HMGA2 reverse | ACTTGTTGTGGCCATTTCCT | |
β-actin forward | ATTGGCAATGAGCGGTTC | 11 |
β-actin reverse | GGATGCCACAGGACTCCAT | |
has-let-7c-RT | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACAACCAT | |
has-let-7c forward | GGCCATGAGGTAGTAGGTTGT | 21 |
has-miR-99a-RT | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCACAAG | |
has-miR-99a forward | GGCAAAACCCGTAGATCCGAT | 21 |
has-miR-199a-3p-RT | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACTAACCA | |
has-miR-199a-3p forward | GGCACACAGTAGTCTGCACAT | 21 |
U48-RT | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACTCAGCG | |
has-U48 forward | CGGCGGTAACTCTGAGTGTGT | 21 |
miRNA reverse | GTGCAGGGTCCGAGGT | |